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cardiac muscle tissue sheets  (Nikon)
95
Nikon cardiac muscle tissue sheets
The combination of isoproterenol, activin A, BMP4, and bFGF induced HAP stem cells in vibrissa hair follicles to form beating <t>cardiac-muscle</t> <t>tissue</t> <t>sheets.</t> (A) Schematic diagram of upper parts of five hair follicles forming cardiac-muscle tissue sheets (upper panel). Bright-field image of upper part of hair follicle differentiatimg to cardiac muscle cells and immunostaining (middle panel). Red = cTnT, Blue = DAPI, Bars = 100 µm. Cardiac-muscle tissue sheet formation process (lower panel). Bar = 500 µm. (B) Immunostaining of beating cardiac-muscle tissue sheets. cTnT-positive cardiac muscle cells were extensively distributed within the sheets. Red = cTnT, Blue = DAPI. Bars = 100 µm.
Cardiac Muscle Tissue Sheets, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cardiac muscle tissue sheets/product/Nikon
Average 95 stars, based on 1 article reviews
cardiac muscle tissue sheets - by Bioz Stars, 2026-04
95/100 stars
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biostation  (Nikon)
96
Nikon biostation
The combination of isoproterenol, activin A, BMP4, and bFGF induced HAP stem cells in vibrissa hair follicles to form beating <t>cardiac-muscle</t> <t>tissue</t> <t>sheets.</t> (A) Schematic diagram of upper parts of five hair follicles forming cardiac-muscle tissue sheets (upper panel). Bright-field image of upper part of hair follicle differentiatimg to cardiac muscle cells and immunostaining (middle panel). Red = cTnT, Blue = DAPI, Bars = 100 µm. Cardiac-muscle tissue sheet formation process (lower panel). Bar = 500 µm. (B) Immunostaining of beating cardiac-muscle tissue sheets. cTnT-positive cardiac muscle cells were extensively distributed within the sheets. Red = cTnT, Blue = DAPI. Bars = 100 µm.
Biostation, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biostation/product/Nikon
Average 96 stars, based on 1 article reviews
biostation - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

Image Search Results


The combination of isoproterenol, activin A, BMP4, and bFGF induced HAP stem cells in vibrissa hair follicles to form beating cardiac-muscle tissue sheets. (A) Schematic diagram of upper parts of five hair follicles forming cardiac-muscle tissue sheets (upper panel). Bright-field image of upper part of hair follicle differentiatimg to cardiac muscle cells and immunostaining (middle panel). Red = cTnT, Blue = DAPI, Bars = 100 µm. Cardiac-muscle tissue sheet formation process (lower panel). Bar = 500 µm. (B) Immunostaining of beating cardiac-muscle tissue sheets. cTnT-positive cardiac muscle cells were extensively distributed within the sheets. Red = cTnT, Blue = DAPI. Bars = 100 µm.

Journal: Cell Cycle

Article Title: Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets

doi: 10.1080/15384101.2016.1146837

Figure Lengend Snippet: The combination of isoproterenol, activin A, BMP4, and bFGF induced HAP stem cells in vibrissa hair follicles to form beating cardiac-muscle tissue sheets. (A) Schematic diagram of upper parts of five hair follicles forming cardiac-muscle tissue sheets (upper panel). Bright-field image of upper part of hair follicle differentiatimg to cardiac muscle cells and immunostaining (middle panel). Red = cTnT, Blue = DAPI, Bars = 100 µm. Cardiac-muscle tissue sheet formation process (lower panel). Bar = 500 µm. (B) Immunostaining of beating cardiac-muscle tissue sheets. cTnT-positive cardiac muscle cells were extensively distributed within the sheets. Red = cTnT, Blue = DAPI. Bars = 100 µm.

Article Snippet: Beating cardiac muscle cells and cardiac-muscle tissue sheets were visualized and recorded with a BioStation IM-Q (Nikon, Tokyo, Japan) and video microscope camera (ScopPad-500, GelleX, Tokyo, Japan).

Techniques: Immunostaining

Flow cytometry analysis of beating cardiac-muscle tissue sheets. (A) Flow cytometry analysis of harvested cardiac-muscle tissue sheets showed that the sheets contain 38.88 ± 6.64% cTnT-positive cells (left panel); 22.19 ± 6.36% CD31-positive cells (middle panel); and 29.58 ± 6.11% SMA-positive cells (right panel). (B) Intercellular Ca2+ imaging of cardiac-muscle tissue sheets. Phase contrast image of cardiac muscle cells loaded with Flou-3 (white dashed area). Images were obtained every 150 msec (left panel). Bar =100 µm. Fluo-3 image at 4 points (I, II, III, IV) showing time course of Fluo-3 intensity change (middle and left panel). (C) High rate of formation of the beating cardiac-muscle tissue sheets from HAP stem cells. Hair follicles were cultured in medium supplemented with isoproterenol, activin A, BMP4 and bFGF, where cardiac-muscle tissue sheets formed at 65.0 ± 5.77%. No cardiac tissue sheets formed in non-supplemented medium or medium supplemented with isoproterenol alone.

Journal: Cell Cycle

Article Title: Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets

doi: 10.1080/15384101.2016.1146837

Figure Lengend Snippet: Flow cytometry analysis of beating cardiac-muscle tissue sheets. (A) Flow cytometry analysis of harvested cardiac-muscle tissue sheets showed that the sheets contain 38.88 ± 6.64% cTnT-positive cells (left panel); 22.19 ± 6.36% CD31-positive cells (middle panel); and 29.58 ± 6.11% SMA-positive cells (right panel). (B) Intercellular Ca2+ imaging of cardiac-muscle tissue sheets. Phase contrast image of cardiac muscle cells loaded with Flou-3 (white dashed area). Images were obtained every 150 msec (left panel). Bar =100 µm. Fluo-3 image at 4 points (I, II, III, IV) showing time course of Fluo-3 intensity change (middle and left panel). (C) High rate of formation of the beating cardiac-muscle tissue sheets from HAP stem cells. Hair follicles were cultured in medium supplemented with isoproterenol, activin A, BMP4 and bFGF, where cardiac-muscle tissue sheets formed at 65.0 ± 5.77%. No cardiac tissue sheets formed in non-supplemented medium or medium supplemented with isoproterenol alone.

Article Snippet: Beating cardiac muscle cells and cardiac-muscle tissue sheets were visualized and recorded with a BioStation IM-Q (Nikon, Tokyo, Japan) and video microscope camera (ScopPad-500, GelleX, Tokyo, Japan).

Techniques: Flow Cytometry, Imaging, Cell Culture